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Contact

Fabien Kawecki

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Contact

Linkedin biotis-bordeaux

Secretary Email

33 (0)5 57 57 14 88

Bioingénierie Tissulaire (BioTis)       

Physical Address:

Batiment BBS (Bordeaux Biologie Santé), 5e étage

2, rue du Dr Hoffmann Martinot,

33000, Bordeaux, France

Mailing Address:

Université de Bordeaux, Campus Carreire

146, rue Léo Saignat, Case 84,

33076, Bordeaux Cedex, France

Cardiac Valve Repair Using Cell-Assembled Extracellular Matrix

Abstract

Reference

Project Leader

Pediatric patients with congenital heart valve disease often require multiple reoperations because current valve substitutes—made from synthetic materials or chemically fixed tissues—provoke chronic inflammation, calcification, and structural degeneration, and crucially cannot grow with the child [1-6]. Although recent approaches such as decellularized grafts, tissue-engineered valves, and in situ regenerative scaffolds have improved biocompatibility, none has yet achieved consistent remodeling, durability, and somatic growth, leaving a major unmet clinical need [7-9]. Our research group has developed Cell-Assembled extracellular Matrix (CAM), a fully biological, non-chemically modified material produced by fibroblasts [10].

CAM is biocompatible, non-immunogenic, easily stored, and retains its structural integrity after devitalization [11]. In preclinical models, CAM-based structures remodel slowly without degradation, and our recent work demonstrated that CAM sheets can successfully replace a pulmonary valve leaflet in sheep, showing good coaptation, hemodynamics, and host-cell–driven recellularization [12].

Building on these results, we propose to develop a CAM-based pulmonary valve capable of integrating with native tissue and adapting to the patient’s growth.

▷Soto M E, Salas J L, Vargas-Barron J, Marquez R, Rodriguez-Hernandez A, Bojalil-Parra R, Pérez-Torres I and Guarner-Lans V 2017 Pre- and post-surgical evaluation of the inflammatory response in patients with aortic stenosis treated with different types of prosthesis BMC Cardiovasc Disord 17 1–8

▷Egbe A C, Connolly H M, Niaz T, Yogeswaran V, Taggart N W, Qureshi M Y, Poterucha J T, Khan A R and Driscoll D J 2017 Prevalence and outcome of thrombotic and embolic complications in adults after Fontan operation Am Heart J 18310–7

▷Firdouse M, Agarwal A, Chan A K and Mondal T 2014 Thrombosis and thromboembolic complications in fontan patients: A literature review Clinical and Applied Thrombosis/Hemostasis 20 484–92

▷Van Brakel T J, Schoof P H, De Roo F, Nikkels P G J, Evens F C M and Haas F 2014 High incidence of Dacron conduit stenosis for extracardiac Fontan procedure Journal of Thoracic and Cardiovascular Surgery 147 1568–72

▷Takajo D, Balakrishnan P L and Aggarwal S 2021 Severe conduit stenosis in a patient with Fontan circulation with a Y-shaped Dacron conduit Cardiol Young 31 1693–5

▷Ovroutski S, Ewert P, Alexi-Meskishvili V, Stiller B, Nürnberg J H, Abdul-Khaliq H, Hetzer R and Lange P E 2004 Comparison of somatic development and status of conduit after extracardiac Fontan operation in young and older children European Journal of Cardio-thoracic Surgery 26 1073–9

▷M K, Güzel Y, Kırat B, Önsel İ Ö, Yorgancılar D, Yücel İ K, Zeybek C and Yıldırım İ S 2025 Cryopreserved Aortic Homograft Replacement in Pediatric Patients: A Single-Center Experience with Midterm Follow-Up Children 12 661

▷Neethling W, Brizard C, Firth L and Glancy R 2014 Biostability, durability and calcification of cryopreserved human pericardium after rapid glutaraldehyde-stabilization versus multistep adapt® treatment in a subcutaneous rat model European Journal of Cardio-thoracic Surgery  10.1093

▷Morales D L, Herrington C, Bacha E A, Morell V O, Prodán Z, Mroczek T, Sivalingam S, Cox M, Bennink G and Asch F M 2021 A Novel Restorative Pulmonary Valve Conduit: Early Outcomes of Two Clinical Trials Front Cardiovasc Med 7. 2020.583360

▷Kawecki F, Gluais M, Claverol S, Dusserre N, McAllister T N and L’Heureux N 2022 Inter-donor variability of extracellular matrix production in long-term cultures of human fibroblasts Biomater. Sci. 10 3935–50

▷Magnan L, Kawecki F, Labrunie G, Gluais M, Izotte J, Marais S, Foulc M P, Lafourcade M and L’Heureux N 2021 In vivo remodeling ofhuman cell-assembled extracellular matrix yarns Biomaterials 273 120815

▷Singh M, Roubertie R, Ozturk C, Borchiellini P, Rames A, Bonnemain J, Dutra Gollob S, Wang SX, Naulin J, El Hamrani D, Dugot-Senant N, Gosselin I, Grenet C, L’Heureux N, Roche ET, and Kawecki F. Hemodynamic evaluation of biomaterial-based surgery for Tetralogy of Fallot using a biorobotic heart, in silico, and ovine models, Science Translational Medicine 16 (2024) 1-17