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Contact

Adrien Naveau

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Contact

Linkedin biotis-bordeaux

Secretary Email

33 (0)5 57 57 14 88

Bioingénierie Tissulaire (BioTis)       

Physical Address:

Batiment BBS (Bordeaux Biologie Santé), 5e étage

2, rue du Dr Hoffmann Martinot,

33000, Bordeaux, France

Mailing Address:

Université de Bordeaux, Campus Carreire

146, rue Léo Saignat, Case 84,

33076, Bordeaux Cedex, France

Biofabrication of an oral mucosa graft using 3D bioprinting (ANR-22-CE52-0004-01)

Abstract

Reference

Project Leader

The main objective of the PERIOPRINT project is to fabricate and validate an in vitro model of gingival connective tissue, mimicking the original structure and composition, to be used for clinical grafting. The research hypothesis is that currently available 3D Bioprinting tools enable to fabricate an in vitro construct for viable grafts in realistic clinical conditions. Indeed, the additive biofabrication offers the possibilities of designing the inner structure of the construct to facilitate the tissue integration, of a spatial control of cell distribution (layer by layer), of producing anatomical design from the patient imaging, of a reproducible and fast production, and of printing in vivo in the future.  

The major scientific barrier of this project is the risk of hypoxia and necrosis in large transplanted constructs, as oxygen and nutrient diffusions to the inner cells are limited. Indeed, initial survival depends on the rapid development of an adequate blood irrigation after implantation. The technical barriers to be lifted are the feasibility of combining in 3D the design of vertical channels for diffusion in the inner structure and horizontal layers with prevascularized networks, both in order to maintain cell survival after in vivo transplantation. The expected results are to obtain the proof-of-concept of a viable gingival connective tissue grafting in vivo, to be used for a clinical approach. With the use of 3D bioprinting enhancing cell viability, and a short maturation of the multi-layered oral graft before implantation, this project could lead to an alternative to autologous grafting.

▷Smirani R, Remy M, Devillard R, Naveau A. Use of human gingival fibroblasts for pre-vascularization strategies in oral tissue engineering. Tissue Eng Regen Med. 2022 Jan 20. doi: 10.1007/s13770-021-00415-3.

▷Oliveira H, Médina C, Stachowicz M-L, et al. 2021. Extracellular matrix (ECM)-derived bioinks designed to foster vasculogenesis and neurite outgrowth: Characterization and bioprinting. Bioprinting. 22:e00134. doi:10.1016/j.bprint.2021.e00134.

▷Smirani R, Remy M, Devillard R, Naveau A. Engineered pre-vascularisation for oral tissue grafting: a systematic review. Tissue Eng Part B 2020, 26(4):383-398. doi: 10.1089/ten.teb.2020.0093

▷Naveau A, Smirani R, Remy M, et al. Cybergology and Bioprinting : the biotechnological future of maxillofacial rehabilitation. Int J Maxillofac Prosthetics 2019 (2):20-26. doi :10.26629/ijmp.2019.05

▷Lei Y, Zouani OF, Rémy M, Ayela C, Durrieu MC. Geometrical microfeature cues for directing tubulogenesis of endothelial cells. PLoS One. 2012;7(7):e41163. doi: 10.1371/journal.pone.0041163.

▷Thebaud NB, Bareille R, Remy M, Bourget C, Daculsi R, Bordenave L. Human progenitor-derived endothelial cells vs. venous endothelial cells for vascular tissue engineering: an in vitro study. J Tissue Eng Regen Med. 2010 Aug;4(6):473-84. doi: 10.1002/term.261. PMID: 20112278.

Camille Déchelette

Collaborator